PepSep Eight Columns have a length of 8 cm. As a shorter column, faster separation gradients can be used, yielding results up to three times faster than columns used in standard proteomics workflows. These columns would be an excellent choice for high-throughput applications.
PepSep Eight Columns are available in different inner diameters, particle sizes and inlets. The columns with a wider ID are a good choice to reduce backpressure, which can accelerate HPLC valve wear, overpressure HPLC systems and potentially cause column damage.
For smooth, safe, and easy connections to the high-pressure end of the column (often via transfer line) the columns are equipped with different connectors:
- patented female ultra-high pressure (UHP) connector. The UHP connection is tested to be used up to 1000 bar.
- or PepSeal connector. It is a 10-32 thread female union which enables easy, precise and fast connection to most LC systems with 1/16 connectors.
On the columns low pressure end, the columns are equipped with the patented male nanoConnect (nC) connector, ensuring a zero dead volume connection. By design, the nanoConnect system makes union and emitter connections quick, reliable, and straightforward.
| Stationary phase | C18 Sapphir | C18 ReproSil AQ | C18 ReproSil AQ |
| Particle size | 1.5 µm | 1.9 µm | 3.0 µm |
| Pore size | 100 Å | 120 Å | 120 Å |
| Surface area | 400 m²/g | 300 m²/g | 300 m²/g |
| pH range | 2-9 | 2-8 | 2-8 |
| Length | 180 mm | ||
| Inner diameter | 50, 75, 100 or 150 µm | ||
| Column type | RP | ||
| Max. Temperature | 60 °C | ||
| Max. Pressure | 1000 bar | ||
| Inlet | UHP or PepSeal | ||
| Outlet | without | ||
