PepSep Twenty-five Columns have a length of 25 cm. These columns would be an excellent choice for routine analysis requiring high sensitivity.
PepSep Twenty-five Columns are available in different inner diameters, particle sizes and inlets. The columns with a wider ID are a good choice to reduce backpressure, which can accelerate HPLC valve wear, overpressure HPLC systems and potentially cause column damage.
For smooth, safe, and easy connections to the high-pressure end of the column (often via transfer line) the columns are equipped with different connectors:
- patented female ultra-high pressure (UHP) connector. The UHP connection is tested to be used up to 1000 bar.
- or PepSeal connector. It is a 10-32 thread female union which enables easy, precise and fast connection to most LC systems with 1/16 connectors.
On the columns low pressure end, the columns are equipped with the patented nanoConnect (nC) connector, ensuring a zero dead volume connection. By design, the nanoConnect system makes union and emitter connections quick, reliable, and straightforward.
|Stationary phase||C18 Sapphir||C18 ReproSil AQ||C18 ReproSil AQ|
|Particle size||1.5 µm||1.9 µm||3.0 µm|
|Pore size||100 Å||120 Å||120 Å|
|Surface area||400 m²/g||300 m²/g||300 m²/g|
|Inner diameter||50, 75, 100, 150 or 200 µm|
|Max. Temperature||60 °C|
|Max. Pressure||1000 bar|
|Inlet||UHP or PepSeal|